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Abstracts of Papers Presented at the 65th Research Meeting of The Medical Research Centre of Bombay Hospital on Monday, 8th March 1999, 2.30 pm in The SP Jain Cafeteria
(Convenor Dr. HL Dhar)

1. LIGHT MICROSCOPY AND HISTOCHEMISTRY OF MUSCLE IN TWO UNUSUAL CASES OF MYOPATHY

Rashna S Dastur, Daya K Manghani, Darab K Dastur

The first case was that of a 73 year old male with proximal muscle weakness. A muscle biopsy of the left quadriceps showed mainly fibro-fatty tissue. Only one area revealed about 3-4 remaining muscle fascicles. The changes seen in this limited amount of muscle tissue, included loss of muscle fibres; infiltration by chronic inflammatory cells; some vasculitis and presence of new formed blood vessels in fibro-fatty matrix; suggesting this to be a form of severe chronic polymyositis. This was consistent with the long duration of 6 years of proximal muscle weakness.

The second case was that of a 39 year old male who had fractured his right shoulder in an accident two years back. There was weakness in the right arm but left arm being normal. One and a half year later he presented with weakness in the left proximal upper limb also. The left deltoid muscle was biopsied. The overall histopathologic appearance were those of a myopathy with excessive inflammatory reaction. The marked variation of fibre size including the very large round hypertrophic fibres along with the excessive inflammatory reaction indicated this to be a case of "Scapulo-humoral type of muscular dystrophy". The severe changes in the left deltoid muscle did not seem to be related to the injury to the right shoulder.


2. IMMUNOHISTOCHEMISTRY FOR MACROPHAGE FACTOR IN BRAIN TUBERCULOMA AND IN INFARCTION; CORRELATION WITH HISTOPATHOLOGY

Pradnya Gaitonde, DK Dastur, DK Manghani

Immuno-histochemical staining is commonly used to determine the cell lineage of a particular cell type. Macrophage factor is one of the markers which labels human monocytes and macrophages and also identifies a population of cells of mononuclear phagocytic origin. This monoclonal antibody also demonstrates the giant cells of macrophagic origin. Hence, this antibody was used on two different cases (i) A case presenting with left front-parietal SOL showed cellular non-neoplastic tissue and reactive astrocytes on histology. Immuno-staining with macrophage factor showed this cellular tissue to be made up of macrophages (gitter cells) consistent with an infarct of short duration and no other inflammatory or neoplastic process. (ii) The specificity of this marker was confirmed on a histologically proven case of a cerebral tuberculoma in which large mononuclears and Langhans’ type giant cells were seen to be positive for macrophage antibody.


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